Figure 6. Overexpression of sirt1 increased protective effects against hydrogen peroxide in human keratinocytes.
HaCaT keratinocyte was transfected by overexpressing adenovirus (Ad-Sirt1) or lacZ-bearing adenovirus (Ad-lacZ) and then exposed to 800 μM H2O2. Cell viability was measured by the Crystal violet assay. Viability of the control cells was set at 100%, and viability relative to the control was measured A. B. LDH activities were measured by quantifying LDH released in the medium C. HaCaT keratinocyte was transfected by overexpressing adenovirus (Ad-Sirt1) or lacZ-bearing adenovirus (Ad-lacZ) for 24h. A Western blot for sirt1 and acetyl-p53 proteins was conducted from HaCaT keratinocytes. Beta-actin was used as the loading control D. Bar graph indicates the mean ± standard error of the mean (SEM) (n = 4). * p < 0.05, ** p < 0.01 significant differences between control and each treatment group.