Figure 8. Selective depletion of Clostridiales Ruminococcaceae by RegIIIγ treatment.
DNA from cecal contents was isolated from Il22−/− mice used in Figure 7, and used as a template for bacterial 16S rDNA PCR analysis, as described previously (63). Amplicons were sequenced using Illumina MiSeq, and analyzed for operational taxonomic units (OTUs) at the phylum, class, order and family levels. A. Total number of OTUs defined as species in PBS versus RegIIIγ treatment groups (top), and the distribution of major phyla (bottom). B. Total number of individual species that were changed by ≥2-fold in response to RegIIIγ treatment categorized by phyla or order as indicated (top), and total number of individual species within Clostridiales that were changed by RegIIIγ treatment categorized by family, as indicated (bottom). Data are combined from two experiments with n=8-10.