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. 2016 May 25;36(6):1174–1185. doi: 10.1161/ATVBAHA.115.306983

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© 2016 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 3. — Artery tertiary lymphoid organs (ATLOs) harbor a diverse repertoire of B-cell subsets. A, Flow cytometry of CD19⁺ B cells of total CD45⁺ leukocytes in abdominal aorta of 80-week-old wild-type (WT) and ApoE^−/− mice (WT, n=11; ApoE^−/−, n=10). B, Immunofluorescence staining with anti-B220 antisera shows B cells in ATLOs and lymph nodes (LNs) but none in the media (M) or plaque (P) or in WT adventitia. C, B cells in the spleen, renal LNs (RLNs), and blood of WT and ApoE^−/− mice (WT, n=8; ApoE^−/−, n=6). Flow cytometric analysis of IgM⁺IgD⁻, IgM⁺IgD⁺, IgM⁻IgD⁻, and IgM⁻IgD⁺ B cells per total CD19⁺ B cells in abdominal aorta (D) and spleen, RLN, and blood of 80-week-old WT and ApoE^−/− mice (WT n=4; ApoE^−/− n=5; E). Percentages of IgM⁺IgD⁺ B cells (F) and IgM⁻IgD⁻ B cells per total B cells in ATLOs, spleen, RLN, and blood of age-matched WT and ApoE^−/− mice (G). Results represent mean±SEM; *P<0.05, **P<0.01, and ***P<0.001; 2-sided unpaired Student t test. n indicates the number of experiments; n.d., not detectable; ns, not significant; and SSC, side scatter.