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. Author manuscript; available in PMC: 2016 Jun 2.
Published in final edited form as: Cancer Cell. 2011 Jun 14;19(6):728–739. doi: 10.1016/j.ccr.2011.05.011

Figure 4. Inflammatory infiltrates in mPanIN lesions and mPDAC in K-RasG12V expressing adult mice treated with caerulein for three months.

Figure 4

(A) K-Ras+/G12V;Elas-tTA/tetO-Cre mice were exposed to doxycycline (thin line) and caerulein (grey box) for the indicated periods of time. Expression of the K-RasG12V oncogene (open box) is indicated. Mice were sacrificed at 8 and 14 month of age.

(B,C) Immunostaining of inflammatory cells surrounding (B) mPanIN lesions in 8 month old mice (2 months after cessation of caerulein) and (C) mPDAC in 14 month old mice (8 months after cessation of caerulein treatment) using antibodies against T lymphocytes (CD3 antibodies), B lymphocytes (Pax5 antibodies), macrophages (F4/80 antibodies) and neutrophils (MPO antibodies). Insets show detailed areas containing the corresponding immune cells. Scale bars represent 50 μm.