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. 2016 May 15;30(10):1240–1250. doi: 10.1101/gad.279414.116

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© 2016 Lee et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Bcl-x_L restrains Bak to inhibit platelet death. (A) Bak^+/Q75L and Bak^Q75L/Q75L mice had significantly decreased platelet numbers compared with Bak^+/+ mice. n = 20. (B) Platelets harboring the Q75L mutation have a reduced life span. n = 8 for Bak^Q75L/Q75L; n = 4 for Bak^+/Q75L. t_1/2 is indicated by a dotted line. Platelets from wild-type mice have a t_1/2 of ∼5 d. n = 4. (C) The effect of the Q75L mutation on platelet numbers is not due to impaired megakaryopoiesis, as the number of mature megakaryocytes was elevated in both Bak^+/Q75L and Bak^Q75L/Q75L mice compared with wild-type mice. n = 12. (D) Loss of Bax resulted in increased platelet counts in Bak^+/Q75L and Bak^Q75L/Q75L mice. n = 3–8. (E) Loss of Bim has no effect on platelet levels in platelets harboring the Q75L mutation (n = 4–17), although loss of Bim alone resulted in thrombocytopaenenia, as previously observed. Data are presented as mean ± SEM. (****) P < 0.0001; (**) P < 0.01; (*) P < 0.05, Student's unpaired t-test.