Fig. 1.
Arrhythmia and simultaneous 2-photon imaging of mitochondrial membrane potential (ΔΨm) in isolated hearts during ischemia-reperfusion. A: percentage of hearts from exercised (Ex) and sedentary (Sed) rats that transitioned to arrhythmia (ventricular tachycardia/ventricular fibrillation) following 40 min of ischemia. B and C: baseline tetramethylrhodamine methyl ester (TMRM) fluorescence (ΔΨm) values were used to normalize all data (F/F0) during ischemia (B) and reperfusion (C). Values (means ± SE) are expressed as percentage of the population for arrhythmia (n = 7–8 per group). *P < 0.05 vs. Sed; #P < 0.05 vs. Sed main effect. D: representative images of ΔΨm in the ventricular free wall and simultaneous ECG recordings during reperfusion for Sed and Ex. reox, Reoxygenation.