Figure 7. Ectopic expression of miR-326 in A549 and SPC-A-1 cells reduces cell migration and invasion motility.
(A) Shown are representative photomicrographs of “wound healing assay” in A549 and SPC-A-1 cells after transfected miRNAs for 0 hour and forty eight hours. Bar = 50 μm. (B) Statistical analysis of “wound healing assay”. (C) A549 and SPC-A-1 cells were loaded onto the top well of a transwell inserts for cell migration or invasion assay. After twenty four hours, cells that migrated to the bottom chamber containing serum-supplemented medium were stained with 0.1% crystal violet, visualized under a phase-contrast microscope, and photographed. Bar = 50 μm. (D) Total number of cells in five fields was counted manually. (E) Expression of MMP-7 and MMP-9 protein in A549 and SPC-A-1 cells after transfection. (F–G) Expression of MMP-7 and MMP-9 mRNA in A549 and SPC-A-1 cells after transfection. Assays were performed in triplicate. Means ± SEM was shown. Statistical analysis was conducted using student t-test.