Figure 1. Identification of miR-200 family and miR-203 as candidate genes for suppression of invasion and/or EMT in HNSCC.
A. Schematic representation of miRNA expression profiles between parent cells (MSCC-1) and a highly invasive clones (MSCC-inv1). MSCC-inv1 cells were isolated from MSCC-1 cells by in vitro invasion assay. MSCC-inv1 cells are spindle shaped, while MSCC-1 cells are cobblestone-like shaped. The miRNA expression profile was examined by microarray. The table shows the top five downregulated miRNAs in MSCC-inv1 cells in comparison with MSCC-1 cells. B. Expression of the top five downregulated miRNAs in MSCC-inv1 cells was confirmed by real-time PCR. The graph shows the expression of these miRNAs (miRNA/U6) in MSCC-1 and MSCC-inv1 cells. All results are presented as means ± SD. *P < 0.05.