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. 2016 May 27;6:27004. doi: 10.1038/srep27004

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Copyright © 2016, Macmillan Publishers Limited

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(A) Phase-contrast image of control ONS cells in normal growth medium. (B) Phase contrast image of control ONS cells grown in cytochalasin (D) neuron-induction medium to generate immature neurons with axon-like processes. (C–E) The same field of undifferentiated ONS cells labelled with an antibody to acetylated α-tubulin (red, C) and phalloidin labelling of actin (green, D), and merged with nuclei labelled with DAPI (blue, E). (F–H) The same field showing a differentiated cell labelled with an antibody to acetylated α-tubulin (red, F) and phalloidin labelling of actin (green, G), and merged with nuclei labelled with DAPI (blue, H). In ONS cells microtubules and actin filaments overlap. Differentiated cells have microtubules but the actin filaments are depleted. Scale bar in (B) applies to (A,B) 100 μm. Scale bar in (H) applies to (C–H) 20 μm.