FIGURE 6.
Emodin inhibits LPS/IFNγ- and IL4-induced activation of signaling pathways. A and B, macrophages were stimulated with (A) LPS (100 ng/ml) and IFNγ (20 ng/ml) or (B) IL4 (10 ng/ml) with or without emodin (Em, 50 μm) for 24 h. Cells were lysed, and cytoplasmic and nuclear fractions were collected. Transcription factors were then detected in both fractions using Western blotting. Bottom panels, quantifications of blots of nuclear fractions normalized to the loading control (Con) TATA-binding protein (TBP). Results are shown as the mean ± S.E. for two independent experiments (n = 4). *, p < 0.05; **, p < 0.01; ***, p < 0.001.