Figure 3. ACs transduced with ETS-TFs express multiple EC markers within 3–4 weeks.
(a) Surface expression for three EC proteins was assayed for by FACS at 7 d (left) and 21 d (right) after lentiviral transduction with ETS-TFs. Within 7 d, differential expression levels of VE-cadherin, VEGFR2 and CD31 are observed. By day 21 of the reprogramming platform, the majority of the cells express high levels of all three EC markers. A minimum of 1 × 105 cells were analyzed for each sample, and all samples were run in triplicate. Error bars depict s.e.m. (b) Immunostaining at days 0 (left images) and 21 (right images) of the reprogramming platform shows substantial accumulation of VE-cadherin protein at the membrane of transduced cells after 21 d (bottom right, green). DAPI was used to stain nuclei (blue). Scale bars, 200 μm.