Extended Data Figure 6. MSI2 OE enhances HSC activity after ex vivo culture.
a, Experimental layout to measure changes in HSC engraftment capacity and frequency with ex vivo culture. b, Representative flow plots of CD45+ GFP+ reconstitution from mice receiving the highest cell dose transplanted per time point. c, Multilineage engraftment of mice injected with D3 cultures. d, Proportion of the human CD45+ graft containing GFP+ cells from D3 primary mice relative to pre-transplant levels of GFP+ cells before expansion. Shown are mice transplanted at the two highest doses (n=8 mice for both conditions). e, Proportion of the human CD45+ graft containing GFP+ cells from D10 primary mice relative to pre-transplant levels of GFP+ cells after expansion. Shown are mice transplanted at the two highest doses (n=8 mice for both conditions, one sample t-test, no change = 1). f, Summary of multilineage engraftment from mice in c. g, GFP mean fluoresence intensity (MFI) in D10 primary engrafted mice. Shown are mice transplanted with the highest three doses, n=11 control and 13 MSI2 mice. h, CD34 expression in GFP-high (top 60%) relative to GFP-low (bottom 40%) gated cells (set per mouse) from engrafted recipients in c. i, Number of transduced phenotyped HSCs after 7 days of culture from HSC expansion experiment described in a. Symbols represent individual mice and shaded symbols represent MSI2 OE. All data presented as mean ± SEM.