Figure 2. Unique and shared enhancers in human ILC and Th subsets.
(A) The stacked bar graph shows numbers of active enhancers that were either unique (gray) or shared (red shades) by the indicated number of cell types. The pie graphs depict cell type distributions for unique (bottom) or shared (top) active enhancers.
(B) Fold change expression of genes (+ SD) located most proximally to enhancers uniquely active in the indicated cell type. For each panel, expression in the indicated cell type was compared to average expression in the other three subsets. *Denotes statistical significance (p<0.01) using a one-way anova.
(C) UCSC Genome Browser views of the human IFNG locus, showing tracks of DNAse-, ATAC- and H3K27ac ChIP-seq data for the indicated cell types, displayed as reads per million (RPM) values. For human Th1 cells, published DNAse-seq data are shown (GSE29692). A track for mammalian sequence conservation is provided at the bottom. Colored bars above tracks represent statistically significant called peaks. Known IFNG regulatory regions (CNSs) are boxed and categorized by cell type restriction in their activities.
(D) Activity status (poised versus off) in other cell types for enhancers that were designated as uniquely active in a given subset. Boxes highlight the subset of enhancers that are off in other cell types.