Figure 2. HBD3-C15 and Nystatin (Nys) showed fungicidal activity against C. albicans. C. albicans (6 × 10⁶ cells/mL) was incubated for 48 hr on cover glass and treated with either HBD3-C15 (0, 12.5, 25, 50, 100, 150, 200, 300 µg/mL), aqueous calcium hydroxide (CH, 100 µg/mL), and Nys (20 µg/mL) for 7 days. (a) FilmTracer LIVE/DEAD Biofilm Viability staining was performed and observed under CLSM; (b) The numbers of dead cells (PI stained red) were increased in the group of HBD3-C15 in a dose-dependent manner; (c) The dead cell biomass were significantly higher in the groups of HBD3-C15 (≥ 100 µg/mL) and Nys than CH group (p < 0.05).