Fig. 3.
Knockdown of Notch2 increases Notch1 activation in MDA-MB-231 xenografts. a Immunoblotting for transmembrane Notch1 and Notch2 and cleaved Notch1 and Notch2, and β-Actin proteins using tumor lysates from MDA-MB-231 xenografts of different groups. Arrow points to cleaved Notch2 band. b Quantitation of proteins shown in panel a Results shown are mean expression of proteins (arbitrary unit) with their 95% confidence intervals (error bars, n = 4 for Control sh-Veh; n = 3 for Control sh-WA; n = 5 for Notch2 sh-Veh; n = 5 for Notch2 sh-WA). Statistical significance of difference was analyzed by generalized linear mixed model and the least square means were used for comparison. The P values were adjusted using Holm-Sidak methods. All P values were two-sided and a significance level was set at 0.05.