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. 2016 May 9;6:25602. doi: 10.1038/srep25602

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(a) CD163/CD14 ratio in TAMs. Human monocytes were isolated from normal donor buffy coat using anti-CD14 magnetic beads. Monocytes were cultured in the presence of M-CSF for 7 days. TAMs, M1 and M2 macrophages were differentiated as described in the Materials and Methods. Characterization of subtypes of macrophages with anti CD-14 FITC (green) and anti CD-163 PE (red) was detected by High Throughput Connotation of Imaging System. (b) Cytokines profiles. After induction of TAMs, M0, M1 and M2 macrophages, levels of IL-12, IL-1β and IL-10 were measured by real-time RT-PCR. The expression of mRNAs was normalized to GAPDH. *P < 0.05; **P < 0.01.