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. Author manuscript; available in PMC: 2017 May 5.
Published in final edited form as: Cell. 2016 Apr 7;165(4):936–948. doi: 10.1016/j.cell.2016.03.026

Figure 3. The neuronal rGC GCY-8 functions downstream of glial KCC-3.

Figure 3

(A) GCY-8 domain structure. (B) Histogram details as in 1B and 1F. *p=0.34. (C) Partial alignment of GCY-8. Red, conserved glycine mutated in gcy-8(ns335). (D,F,H) Fluorescence, TEM, and FIB-SEM reconstruction, respectively, of gcy-8(ns335) AFD receptive-ending. (E, G) TEM and FIB-SEM reconstruction, respectively, of wild-type AFD receptive-ending. (D) Arrow, dendritic shaft. Arrowhead, short microvilli. (E,F) Arrowheads, microvilli. Asterisk, dendritic shaft. Arrow, glial cytosol. Scale bar, 1µm. (I) Histogram details as in 1B. (J, K) Thermotaxis assays as in 1G. Animals raised at 15°C (J) or 25°C (K). (L) Histogram as in 1B. Hatched, animals with disorganized microvilli. See also Figure S3, S4 and Movies 1–4.