Figure 3. Dependence of cell size oscillation on a, and oscillations in gene expression.
a, Dependence of the probability of oscillation on a. For each value of a, 200 simulations were run using the rescaled linear map (Eq. 2). Closed symbols indicate analysis of our own experimental data (MC4100 grown at 37°C, 27°C and 25°C, red circles from left to right (n = 143, 48, and 57, respectively)) and previously published data (MG1655, blue square (n = 97); B/r strain, blue triangle (n = 60)). Only lineages without aberrant cell cycles are shown (Methods). The horizontal error bars indicate the 95% confidence interval.
b, Dependence of the average oscillation frequency on a using the same data set presented in a. Only lineages that were considered oscillatory were used for the calculation (n = 51 (37°C), 18 (27°C), and 36 (25°C) for MC4100, 34 for MG1655, and 23 for B/r strain). The shaded region represents standard deviation. As in a, closed symbols indicate analysis of experimental data; the vertical error bars indicate standard deviation.
c, Distributions of oscillation frequencies from experimental (MC4100, 37°C; n = 51) and simulated (n = 80) cell-size dynamics. Simulations were done with a = 0.88 using Eq. 2.
d, Oscillation scores of simulated (black, Methods) and experimental (MC4100 at 37°C, red) YFP concentrations ([Y]). The data were sorted according to the oscillation score and shown in an ascending order. The dashed line indicates a threshold for the oscillation score (Methods).
e, ACFs of [Y] for four different frequencies. For each frequency (indicated in each panel), the sample with the highest score (above the threshold) is shown for simulation (black) and experiment (red).
f, Distributions of oscillation frequencies in [Y] for simulation (black, n = 134) and experiment (red, n = 108).