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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: AIDS. 2016 May 15;30(8):1163–1174. doi: 10.1097/QAD.0000000000001079

Fig. 4. TA-cloning and Sanger sequencing of representative samples confirmed the deletion of predicted fragments between corresponding gRNA target sites (A-C) and various additional insertions (B, C).

Fig. 4

The PAM sequences are highlighted red and the scissors indicate the third nucleotide from PAM. The red arrow points the junction site after cleavage and ligation.