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. 2016 Apr 29;6:24185. doi: 10.1038/srep24185

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Total RNA was isolated from U87 cells that were treated with either vehicle or LY500307 for 48 h using RNeasy mini kit and the quality of RNA was tested using a Bio-analyzer and subjected to RNA sequencing. (A) heat map of differentially expressed genes between vehicle and LY500307 is shown. (B) Differentially expressed genes were subjected to pathway analysis using IPA software and the top five associated network functions of differentially expressed genes are shown. (C) The selected top canonical pathways are shown. (D–E) U87 cells that were treated with either vehicle or LY500307 for 48 h and differentially expressed genes that were upregulated or downregulated were validated using qRT-PCR. F, U251 cells were treated with either vehicle or LY500307 for 48 h and differentially expressed genes were validated using qRT-PCR.