Figure 6.
Immunolocalization of PC2 in hypophysiotropic areas of the hypothalamus during fed versus fasted states. (A and B) Typical photomicrographs showing the immunodistribution of PC2 in the PaLMs and the PaMPs. (C and D) The ME. (E and F) The CC. A significant decrease in PC2 immunoreactivity is shown in the PaMPs (the location of hypophysiotropic TRH neurons) from fasted animals (B) as compared with fed controls (A). See also the high-magnification images at the left of A and the right of B. In the same brain section, the PaLM subdivision, which also expresses PC2, showed a decrease during fasting (A and B; see also the high-magnification images). A and B show low magnification (×10, scale bar: 200 μm). High-magnification images are indicated by arrows (×40, scale bar: 50 μm). The ME region (C and D) shows a weak, unchanged immunostaining in the internal zone and less intense staining in the external zone of the medial portion of the ME in fed as compared with fasted animals. No changes were observed in the anterior and posterior ME (not shown). The immunoreactivity for PC2 in the CC (E and F) did not show differences between fed and fasted animals (see also insets). Magnification in E and F, ×20; scale bar: 50 μm.