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. Author manuscript; available in PMC: 2016 Sep 28.
Published in final edited form as: Nat Neurosci. 2016 Mar 28;19(5):725–733. doi: 10.1038/nn.4281

Figure 1. VGluT2 neurons of the VTA make asymmetric synapses on nAcc mShell neurons.

Figure 1

(a) Diagram of virus injection in VTA of VGluT2::Cre mice. (b) VTA neuronal expression of eYFP (green; under vglut2-promoter control) within the VTA containing tyrosine hydroxylase (TH; red). (c and d) Fibers (green) from VTA-VGluT2 neurons are highly concentrated in nAcc mShell. Section counterstained with nuclear dye DAPI (blue). LV, lateral ventricle. (e) Axon terminal (AT) containing mCherry-labeling (scattered dark material) and VGluT2-labeling (gold particles, arrowhead) makes an asymmetric synapse (arrow) with a dendrite (De) in nAcc mShell. Bars: (b) 100 μm, (c) 500 μm, (d) 20 μm, and (e) 200 nm.