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. Author manuscript; available in PMC: 2017 Apr 20.
Published in final edited form as: Neuron. 2016 Apr 7;90(2):278–291. doi: 10.1016/j.neuron.2016.03.011

Figure 6. Presynaptic localization of PFK-1.1 depends of synaptic scaffolding proteins.

Figure 6

(A–N) Localization of PFK-1.1 in GABA ventral neurites (pseudocolored magenta) and its respective pixel fluorescence values along the neurite after 10 minutes of hypoxia in wild-type (A, B); synaptic vesicle kinesin mutant unc-104(e1265) (C, D); mitochondria localization and distribution mutants klp-6(sy511) (E, F), ric-7(nu447) (G, H), and dpr-1(tm408) (I, J); synaptic scaffolding mutant syd-2(ju37) (K, L); and syd-2(ju37) mutant animals expressing a wild-type rescuing array of syd-2 cell-specifically in GABA neurons (M, N).

(O) Percentage of animals displaying PFK-1.1 clusters in GABA neurites in varying genotypes after 0 or 10 minutes of exposure to hypoxic conditions, as indicated. For syd-2(ju37), PFK-1.1 clustering was examined in the absence (−) or in the presence (+) of a wild-type rescuing array of syd-2 cell-specifically in GABA neurons. Number of animals tested is indicated at the bottom of each column.

(P) Quantification of the synaptic enrichment of PFK-1.1, (ΔF/F) in the NSM neurons for wild-type (black circles) and syd-2(ok217) (white circles) under hypoxic conditions. The circles in the graph represent individual animals.

Scale bar: 5μm. Error bars: SEM. *, p < 0.05. **, p < 0.01. ***, p < 0.001 between indicated groups.