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. Author manuscript; available in PMC: 2017 Apr 20.
Published in final edited form as: Neuron. 2016 Apr 7;90(2):278–291. doi: 10.1016/j.neuron.2016.03.011

Figure 3. Glycolysis is required to maintain the synaptic vesicle cycle.

Figure 3

(A–I) Synaptic vesicle marker CAT-1::GFP in NSM serotonergic neurons for wild-type after 10 minutes under hypoxic conditions (A), endocytosis mutant unc-57(ok310)/Endophilin A at normoxic condition (D), and unc-13(e450);pfk-1.1(ola72) double mutants after 10 minutes under hypoxic conditions (G). Ratiometric image of NSM representing the relative enrichment of CAT-1::GFP signal over cytosolic mCherry signal in the respective genotypes (B, E, H) and the respective line scan pixel fluorescence values of the synaptic vesicle marker CAT-1::GFP (C, F, I).

(J) Percentage of animals displaying a diffuse distribution of synaptic vesicle proteins in the NSM neuron after 10 minutes of hypoxia in pfk-1.1(ola72) (gray bar), unc-57(ok310); pfk-1.1(ola72) (gray bar with black outline) and unc-13(e450);pfk-1.1(ola72) (red bar) double mutants. Number of animals scored is indicated at the bottom of each column. pfk-1.1(ola72) is the same as shown in Figure 1J.

(K–L) Percentage of fluorescence recovery after photobleaching (FRAP) at synaptic varicosities of GABA neurons in unc-26(s1710) (triangles), unc-57(ok310) (squares), pfk-1.1(ola72) (gray circles), and wild-type (black circles) animals over time (K); and at 30 seconds post photobleaching (L). Number of animals tested is indicated at the bottom of each column.

Scale bar: 5μm. Error bars: SEM. *, p < 0.05. **, p < 0.01. ***, p < 0.001.