FIGURE 3.
(A) Predicted secondary structures of A. thaliana pre-tRNA substrates used in this study. All substrates were prepared using in vitro transcription. Cys-Mito and Phe-Chlor substrates are derived from the organellar genomes (Cys-mitochondrial and Phe-chloroplast), and Cys-nuclear and Phe-nuclear are from the nuclear genome. The pre-tRNAs contain 5-nt leaders labeled with fluorescein at the 5′ end and a discriminator base at the 3′ end. (B) Model substrates. Predicted secondary structures of two model substrates, ΔAC and SL, derived from the mitochondrial pre-tRNACys sequence. The SL substrate connects the acceptor stem helix and TψC-arm.