Table 4.
Secondary antibodies, dilutions, and excitation/emission rate
| Fluorochrome | Specificity | Dilution | Art.#/company | Excitation/emission |
|---|---|---|---|---|
| FITC | Anti-mouse | 1:150 | Jackson Immuno Research/200-542-037 | 493/519 |
| Cy3 | Anti-mouse | 1:150 | Jackson Immuno Research/200-162-037 | 550/570 |
| Cy3 | Anti-rabbit | 1:150 | Jackson Immuno Research/711-165-152 | 550/570 |
| Cy5 | Anti-mouse | 1:450 | Invitrogen/A10524 | 649/665 |
| Alexa 488-α-BTX | Snake | 1:500 | Anti-nicotinic acetylcholinereceptor | 495/519 |