Figure 1.
TFH and B lymphocyte absolute values in CVID and N. (a) Absolute lymphocyte values (cells/mm3) were calculated in CVID Group I, n = 8 (I), and Group II, n = 13 (II), patients and control donors (N), n = 19. Peripheral blood was stained with monoclonal antibodies and absolute values of viable CD4+ T lymphocytes and CD4+ T cells coexpressing CXCR5 (TFH) were calculated after analysis on a FACScan Becton Dickinson flow cytometer. (b) A comparison between peripheral blood viable B lymphocytes (CD19+ cells), memory B lymphocytes (CD27+CD19+ cells), and TFH values (CXCR5+CD4+ cells) was done in CVID Group I, Group II, and N controls. Lineal regression analysis was consigned in the graphs. Only in Group I was the correlation statistically significant.