. 2015 Nov 25;7(2):1464–1476. doi: 10.18632/oncotarget.6386

Table 1. Antibodies used for IHC analysis.

Markers	Company	Clone	Dilution	Antigen retrival	Incubation condition (min, °C)	Assessment	cutoff
ER	Neomarkers	SP1	Pre-diluted	EDTA pH8	32,37	N	1%
PR	Ventana	IE2	Pre-diluted	EDTA pH8	32,37	N	1%
AR	Dako	AR441	1:100	EDTA pH8	56,37	N	1%
Ki67	Ventana	41912	Pre-diluted	EDTA pH8	32,37	N	14%
EGFR	Ventana	3C6	Pre-diluted	EDTA pH8	32,37	M	5%
HER2	Ventana	4B5	Pre-diluted	EDTA pH8	16,37	M	3+
CK5/6	Dako	D5/16 B4	1:40	EDTA pH8	32,37	C,M	5%
CK14	Neomarkers	LL002	1:100	EDTA pH8	32,37	C,M	5%
c-kit	Dako	104D2	1:300	EDTA pH8	32,37	C,M	5%
P63	Ventana	4A4	Pre-diluted	EDTA pH8	32,37	N	5%
Synaptophysin (SYN)	Novocastra	27G12	1:50	EDTA pH8	32,37	C,M	1%
Chromogranin (CG)	Biogene	MU-126-UC	1:200	EDTA pH8	32,37	C,M	1%
SOX2	Ventana	SP76	Pre-diluted	EDTA pH8	32,37	N	1%
vimentin	Dako	V9	1:2000	EDTA pH8	24, RT	C, M	10%
p-cadherin	BD transduction lab	56/p-cad	1:200	EDTA pH8	32,37	C,M	10%
CD44	Ventana	SP37	Pre-diluted	EDTA pH8	32, 37	M	5%
ALDH	BD transduction lab	44/ALDH	1:600	EDTA pH8	32, 37	C	5%
DCLK1	Abcam	Polyclonal	1:100	Citrate pH6	32, 37	C	IHC score 4

‘N’: nuclear; ‘C’: cytoplasmic; ‘M’: membraneous