Table 2.
Genetic rescue of mutants with the fTRG lines Table listing fTRG lines and respective genetic alleles as well as rescue assays that were used to assess the functionality of the fTRG lines. Note that about two-thirds of the lines rescue the mutant phenotypes.
| Gene | Chromosome | fTRG line | Tag | Rescue? | Rescue assay | Alleles, deficiencies used in trans for rescue assay | Reference |
|---|---|---|---|---|---|---|---|
| amos | 2nd | fTRG_218 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | antenna size and bristle number rescued to normal | amos[3] | |
| anterior open (aop, Yan) | 2nd | fTRG_142 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | embryonic lethality rescued to viable adults | aop[1] (BL-3101); aop[Yan1] (BL-8780) | |
| aubergine (aub) | 2nd | fTRG_581 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | female sterility entirely rescued | aub[HN2] (BL-8517); Df(2L)BSC145 (BL-9505) | |
| baboon (babo) | 2nd | fTRG_444 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality rescued to viable adults | babo[32] (BL-5399); babo[k16912] (BL-11207) | |
| bag of marbles (bam) | 3rd | fTRG_3 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | female sterility entirely rescued |
bam[delta86]; Df(3R)exel9020 |
Christian Bökel, pers. comm. |
| cactus (cact) | 2nd | fTRG_516 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality and female sterility rescued | cact[1]; cact[4] | |
| CG32121 | 3rd | fTRG_92 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | flightlessness rescued |
CG32121 [XZ1] (X. Zhang and F.S., unpublished); Df(3L)ED4502 (BL-8097) | |
| CG6509 (dlg5) | 2nd | fTRG_10251 | 2xTY1-sGFP-3xFLAG | yes | lethality rescued to viable adults | CG6509 [KG006748] (BL13692); Df(2L)BSC244 (BL-9718) | |
| discs large 1 (dlg1) | X | fTRG_502 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | male lethality rescued to viable adults | Dlg1[5] (BL-36280) | |
| dorsal (dl) | 2nd | fTRG_29 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | bristle number rescued to normal | dl[1]; dl[4] | |
| ebi | 2nd | fTRG_10141 | 2xTY1-sGFP-3xFLAG | yes | lethality rescued to viable adults | ebi[CCS-8] (BL-8397); ebi[E90] (BL-30720) | |
| escargot (esg) | 2nd | fTRG_10170 | 2xTY1-sGFP-3xFLAG | no | lethality not rescued | esg[35Ce-1] (BL-3900); esg[35Ce-3] (BL-30475) | |
| eyes absent (eya) | 2nd | fTRG_492 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | no | lethality not rescued | eya[C0233]; eya[C0275] | |
| fat (ft) | 2nd | fTRG_10233 | 2xTY1-T2A-nlsGFP-3xFLAG | yes | lethality rescued to viable adults | ft[G-rv] (BL-1894); ft[8] (BL-5406) | |
| 48 related 2 (Fer2) | 3rd | fTRG_334 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | defective climbing rescued to wild type | Fer2[e03248] | Dib et al., 2014 |
| fizzy (fzy) | 2nd | fTRG_10250 | 2xTY1-T2A-nlsGFP-3xFLAG | no | lethality not rescued | fzy[1] (BL-2492); fzy[3] (BL-25143) | |
| flightless I (fliI) | X | fTRG_467 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality or flightlessness rescued |
fli[14] (BL-7481); fli[3] (BL-4730) | |
| Hand | 2nd | fTRG_10163 | 2xTY1-sGFP-3xFLAG | yes | semi-lethality rescued to viable adults | Hand[173] | |
| hippo (hpo) | 2nd | fTRG_10130 | 2xTY1-sGFP-3xFLAG | yes | larval lethality rescued to viable adults | hpo[KS240] (BL-25085); hpo[KC202] (BL-25090) | |
| HLH54F | 2nd | fTRG_153 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality rescued to viable adults | bHLH54F[598]; Df(2R) Exel7150 (BL-7891) | |
| Integrin linked kinase (Ilk) | 3rd | fTRG_483 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | embryonic lethality rescued to viable adults (wing blisters) | Ilk[1] (BL-4861); Df(3L)BSC733 (BL-26831) | |
| Kinesin heavy chain (Khc) | 2nd | fTRG_10243 | 2xTY1-T2A-nlsGFP-3xFLAG | yes | lethality rescued to viable adults | Khc[8] (BL-1607); Khc[1ts] (BL-31994) | |
| LanB1 | 2nd | fTRG_681 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | lethality rescued to viable adults | LanB1 [KG03456] (BL-13957); Df(2L) Exel7032 (BL-7806) | |
| multiple ankyrin repeats single KH domain (mask) | 3rd | fTRG_486 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality rescued to viable adults |
mask[10.22]/ Df(3R)BSC317 | Barry Thompson, pers. comm |
| midline (mid) | 2nd | fTRG_490 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | lethality not rescued |
mid[B1295]; mid[C2372] | |
| numb | 2nd | fTRG_25 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality rescued to viable adults |
numb[1] (BL-4096); Df(2L)30A-C (BL-3702) | |
| odd skipped (odd) | 2nd | fTRG_47 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | lethality not rescued |
odd[5] (BL-5345); Df(2L) Exel7018 (BL-7789) | |
|
optomotor-blind-related-gene-1
(org-1) |
X | fTRG_485 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | male lethality not rescued | org-1[OJ487] | |
| oskar (osk) | 3rd | fTRG_1394 | 2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | yes | female sterility entirely rescued |
osk[A87]/ Df(3R)p-XT103 |
|
| Pabp2 | 2nd | fTRG_565 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | no | lethality not rescued | Pabp2[01] (BL-9838); Pabp2[55] (BL-38390) | |
| patched (ptc) | 2nd | fTRG_82 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | yes | lethality rescued to viable adults | ptc[9] (BL-3377); ptc[16] (BL-35500) | |
| retina abarrent in pattern (rap) | X | fTRG_1253 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | lethality rescued to viable adults | rap[ie28] | Yuu Kimata, pers. comm. |
| rhea (Talin) | 3rd | fTRG_587 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | embryonic lethality rescued to viable adults | rhea[1]; rhea[79] | |
| RhoGEF2 | 2nd | fTRG_591 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | embryonic lethality rescued to viable adults |
RhoGEF2
[04291] |
Jörg Großhans, pers. comm. |
| roundabout (robo) | 2nd | fTRG_567 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | no | lethality not rescued | robo[1] (BL-8755); robo[2] (BL-8756) | |
| saxophone (sax) | 2nd | fTRG_10070 | 2xTY1-sGFP-3xFLAG | yes | lethality rescued to viable adults | sax[4] (BL-5404); sax[5] (BL-8785) | |
| scribbler (sbb) | 2nd | fTRG_443 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | lethality not rescued | sbb[04440] (BL-11376); Df(2R)BSC334 (BL-24358) | |
| Sin3A | 2nd | fTRG_596 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | no | lethality not rescued | Sin3A[08269] (BL-12350); Sin3A [B0948] | |
|
smoothened
(smo) |
2nd | fTRG_599 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | lethality rescued to viable adults | smo[3] (BL-3277); smo[119B6] (BL-24772) | |
| snail (sna) | 2nd | fTRG_71 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | lethality not rescued |
sna[18]
(BL-2311); sna[1] (BL-25127) |
|
| spalt major (salm) | 2nd | fTRG_165 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | lethality not rescued | salm[1] (BL-3274); Df(2L)32FP-5 (BL-29717) | |
| Target of rapamycin (Tor) | 2nd | fTRG_713 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | no | lethality not rescued | Tor[deltaP] (BL-7014); Df(2L) Exel7055 (BL-7823) | |
| traffic jam (tj) | 2nd | fTRG_163 | 2xTY1-sGFP-V5-preTEV-BLRP-3xFLAG | no | sterility not rescued | tj[PL3] (BL-4987); Df(2L)Exel8041 (BL-7849) | |
| viking (vkg) | 2nd | fTRG_595 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | no | lethality not rescued | vkg[01209] (BL-11003); Df(2L) Exel7022 (BL-7794) | |
| Unc-89/ Obscurin | 2nd | fTRG_1046 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | flightlessness rescued |
Unc-89[EY15484] | |
| yorkie (yki) | 2nd | fTRG_875 | 2xTY1-sGFP-V5-preTEV-BLRP-3XFLAG | yes | lethality rescued to viable adults | yki[B5] | Barry Thompson, pers. comm. |