Figure 3.
Analysis of RNA extracted from ASC-released MVs. (A): Small RNA (fewer than 200 nucleotides) and large RNA extracted from ASCs and ASC-released MVs were quantified (n = 3– 4). Total RNA was set to 100%. (B): Equal amounts of total RNA from ASCs and MVs were analyzed using Bioanalyzer (Agilent Technologies). 18S and 28S indicated the ribosomal RNA. (C): Small RNA from MVs was analyzed using Bioanalyzer. (D): An electropherogram of the lane MV on (C). (E): Reverse-transcriptase-polymerase chain reaction analysis of miRNA for small RNA extracted from MV-P. The level of miRNA in MV was set to 1 (dashed line). U6 was used as an internal control. n = 4. ∗, p < .05; ∗∗, p < .01. Abbreviations: ASC, adipose-derived stem cell; miRNA, microRNA; MV, microvesicle; nt, nucleotide.