Figure 2. Confirmation of the myogenicity of isolated MuSCs.
(A) Immediately following FACS isolation, 5,000 quiescent MuSCs were plated in a well of an 8-well chamber slide that had been coated with Poly-D lysine and ECM. Cells were fixed with 4% paraformaldehyde 6 hours (6 hr), 3 days (3 d) and 5 days (5 d) following plating, and stained with antibodies against Pax7, MyoD1 and Myogenin, respectively. DAPI stains all nuclei. Note the changes in cell size and morphology. Scale bars represent 70 μm in the images and 10 μm in the inserts. (B) Immediately following FACS isolation, 5,000 activated MuSCs or their progeny from limb muscles that had been injured three days earlier were plated in a well of an 8-well chamber slide that had been coated with Poly-D lysine and ECM. Cells were fixed with 4% paraformaldehyde 6 hours later and stained with an anti-MyoD1 antibody. DAPI stains all nuclei. Scale bars represent 70 μm in the image and 10 μm in the insert.