Figure 2. Representative coronal sections immunostained for BrdU and DCX quantification in the SVZ.
Low-magnification images [selected from +1.1 mm anterior to Bregma from an 8-month-old male Abca7−/− mouse (A–C) and an 8-month-old male WT mouse (D–F)] demonstrate the distribution of BrdU (A) and DCX (D) immunoreactivity in representative coronal sections. Proliferating BrdU +ve cells were clearly detected within the wall of the lateral ventricle as illustrated in “A”, and viewed at higher magnifications (rotated 90° clockwise) in “B” and “C”. DCX staining of neuroblasts in a coronal section of the SVZ is presented in panels “D”–“F”. Fine processes with bifurcations were detected as DCX +ve immunostaining (in panel F) in the SVZ. Similar neuroblast morphology was seen in the DG (i.e. compare figure F with Figure 1F). The broken-line arrows point to the corresponding zoomed pictures. Scale bar: for A and D is 500 μm; for B and E is 150 μm; for C and F is 25 μm).