Figure 7.
Survival curves of G. mellonella larvae infected with the A. nosocomialis parent or the ΔkatEΔkatG mutant strain. Overnight bacterial culture were inoculated into fresh medium and allow to grow for 3 h. Cells were collected by centrifugation, washed and resuspended in PBS to a concentration of 2× 107 CFU/ml. Each G. mellonella larva was injected with 1× 105 CFU of the A. nosocomialis parent or the ΔkatEΔkatG mutant strain (in 5 µl PBS), or injected with 5 µl PBS through the last left proleg. Injected larvae were incubated at 37°C in humidified environment. Survival was monitored for 8 days. Survival curves represent data compiled from 4 independent experiments. The survival curves were compared by log-rank test. *, p<0.05.