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DASL Performance as a Function of RNA Degradation Level
| Duration of treatment at 95°C | 0 min | 5 min | 10 min | 20 min | 40 min | 60 min |
|---|---|---|---|---|---|---|
| % RNA detectable by RT—PCR | 100 | 53.02 | 22.90 | 5.47 | 0.75 | 0.13 |
| Correlation between technical replicates, R2 | 1.00 | 1.00 | 1.00 | 0.99 | 0.98 | 0.94 |
| Correlation with undegraded RNA, R2 | 1.00 | 0.91 | 0.83 | 0.77 | 0.71 | 0.61 |
| % of genes detected, compared to undegraded RNA | 100 | 99 | 98 | 97 | 94 | 88 |
