Figure 2. Mitotic index and apoptosis in diploid vs. aneuploid CRC cells.
(A) Representative images of DLD1, DLD1 + 7, and DLD1 + 13 cells grown under standard culture conditions and stained with DAPI. Arrows point to mitotic cells. Images are maximum intensity projections of Z-stacks acquired at 0.6 μm steps. Scale bar, 10 μm. (B,C) Mitotic indices for the three cell lines cultured under different conditions and reported with data grouped either by culture conditions (B) or by cell line (C). The data are reported as mean and standard errors from three biological replicates. Statistical analysis was performed using the χ2 test and only p values that were significant or close to significance are reported in the figure. (D) The same data reported in (C) are shown here as normalized value over the mitotic index of the given cell line cultured under standard conditions. (E) Representative TUNEL assay image of DLD1 + 13 cells cultured under standard conditions. Arrow points to TUNEL-positive cell. Images are maximum intensity projections of Z-stacks acquired at 0.6 μm steps. Scale bar, 10 μm. (F,G) Quantification of TUNEL-positive cells in the three cell lines cultured under different conditions and reported with data grouped either by culture conditions (F) or by cell line (G). The data are reported as mean and standard errors from three biological replicates. Statistical analysis was performed using the χ2 test and only p values that were significant or close to significance are reported in the figure. (H) The same data reported in (G) are shown here as normalized value over the TUNEL-positive values of the given cell line cultured under standard conditions.