Figure 1.
Hypoxia and stabilization of HIF1α occur during PanIN development.
A, Immunohistochemical staining for HIF1α or Hypoxyprobe in pancreata from WT and KrasG12D mice. Tissues are from 2-month-old WT mice with histologically normal pancreas (left), 2-month-old KrasG12D mice with areas of PanINs adjacent to areas of normal pancreas (middle), and moribund (8-22 months) KrasG12D mice with areas of invasive PDAC (right). Dashed lines and arrows denote fibroin flammatory area. Arrowheads indicate PanIN. Corresponding quantification is indicated in the graph (n = 5 WT, n = 5KrasG12D PanIN, n = 4KrasG12D PDAC, n = 3 FOV per animal for HIF1α;n = 4 WT, n = 4KrasG12D PanIN, n = 5KrasG12D PDAC, n = 3 FOV per animal for Hypoxyprobe). FOV, fields of view.
B, HIF1α immunohistochemical staining in representative samples of human normal pancreatic (left), PanIN (middle), and PDAC (right) tissues. Arrowheads indicate PanIN. Corresponding quantification is indicated in the graph (n = 7 normal, n = 24 PDAC).
Scale bars (A,B), 100 μm. The data in A,B are shown as the mean ± s.e.m. P values were determined by Mann-Whitney test. ***P < 0.001, ****P< 0.0001.