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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Cell. 2016 Feb 11;164(4):792–804. doi: 10.1016/j.cell.2016.01.038

Figure 6. Multiplexed detection of mRNAs in CLARITY.

Figure 6

(A) Left, multiplexed in situ hybridization of 0.5mm coronal CLARITY section treated with kainic acid, using somatostatin (red), parvalbumin (blue) and tyrosine hydroxylase (green) probe sets. Scale bar, 500 µm. Middle, inset of caudal hippocampus showing parvalbumin and somatostatin interneurons in CA1 region. Scale bar, 50 µm. Right, parvalbumin and tyrosine hydroxylase positive cells in midbrain. Scale bar, 100 µm.

(B) 3D rendering of 1mm CLARITY block, HCR in situ hybridization for somatostatin (red) and Arc mRNA (green) in control saline injected (left) and kainic acid injected (right) animals. Right, magnified view of indicated boxes. Scale bar, 100 µm. See also Figure S6