Figure 2.
Gene marking in three pigtailed macaques following autologous transplant with Cal-1-transduced CD34+ cells. (a) Total lentiviral vector DNA was quantified by Taqman from animal ID A11199 (blue bars), A12309 (green bars), or A11209 (purple bars) following transplant and SHIV challenge. Arrow indicates SHIV challenge, and open squares indicate time points collected following SHIV challenge. (b) Average gene marking values pre- (black bars) and postinfection (white bars) for each animal. Preinfection values were collected following posttransplant stabilization of platelet counts. 100% gene marking represents 1 copy of vector provirus per cell, based on a standard curve constructed from a single-copy lentivirus-infected cell line. Error bars represent standard error of the mean for at least three independent time points. P values: NS: not significant; *P ≤ 0.05; ****P ≤ 0.0001. SHIV, simian/human immunodeficiency virus.