Figure 4. Progenitors organoids model early PDAC.
(a) Areas of progenitor organoids expressing transgenes. (b) Representative images of mCherry, TP53R175H and KRASG12V expressing structures stained for Ki67 (green), RFP (red) and DAPI (blue). (c) Quantification of the percentage of proliferative organoids. (d) Form factor (FF) analysis, a continuous scale where a perfect circle is represented by FF=1 and a linear line by FF=0. (e) Overlay of DIC and fluorescent images of KRASG12V and TP53R175H expressing structures (top) and sections stained with H&E (bottom panel). All quantification graphs summarize three independent experiments with N >100 structures assessed in all cases, N.S-, not significant; * P = 0.01 – 0.05; ** P = 0.001 – 0.01; ***P = < 0.001 (N = 3 biological repeats). (f) Transplant outgrowths from progenitor organoids expressing mCherry, KRASG12V or TP53R175H. Morphologies of organoid-derived structures are shown by H&E images. Expression of Keratin19 (KRT19, green), KRAS (green) or TP53 (green), DAPI (blue) as well as human marker HLA (red) are shown in immunofluorescent images. All scale bars represent 50 µm.