Figure 4. Effect of NZ51 on cell viability of normal breast cell lines and breast cancer cell lines as well as on cell cycle progression of MCF-7 and MDA-MB-231.
A. Cell viability assay of normal immortalized breast cell lines and breast cancer cell lines incubated with different concentrations of NZ51 for 72 h at 0.001, 0.01, 0.1, 1, 5, 10 and 20 μM. B. Flow cytometry analysis determinations of the percentage of MCF-7 cancer cells in G1, G2/M, and S phase of the cell cycle during three consecutive days of treatment with NZ51 at 0, 4, 8, 12 μM. The latter concentrations are indicated under the bars of each graph. C. Flow cytometry analysis performed with MDA-MB-231 cancer cells.