FIGURE 7.
Characterization of Brpf3−/− mice and MEFs. A, representative hind limb clasping test of wild-type and Brpf3−/− mice. B, wild-type and Brpf3−/− MEFs were monitored by an IncuCyte live cell imaging system. The results were calculated from two pairs of wild-type and Brpf3−/− embryos, with each pair analyzed in triplicates. C and D, RT-qPCR analysis of Brpf1 and Brpf2 transcripts in MEFs derived from E13.5 (C) and E15.5 (D) embryos. RT-qPCR was performed in two pairs of wild-type and Brpf3−/− embryos; for each pair, RT-qPCR in triplicates and average values are shown with standard deviations. E and F, RT-qPCR analysis of Brpf1 and Brpf2 transcripts in the testis and brain of wild-type and Brpf3−/− mice. RT-qPCR was performed in three pairs of wild-type and Brpf3−/− mice; for each pair, RT-qPCR in triplicates and average values are shown with standard deviations. G, RT-qPCR analysis of Moz, Morf, Hbo1, and Tip60 transcripts as in D. **, p < 0.01; ns, not statistically significant. H, immunoblotting of protein extracts from wild-type and Brpf3−/− MEFs (lanes 1 and 2), E12.5 embryos (lanes 3 and 4), and adult testis and brain tissues (lanes 5–8) with different anti-histone antibodies.