Figure 3. XLOC_006390, HOTTIP-005, and RP11-567G11.1 expression were increased in PC cell lines and tissues.

A. qRT-PCR evaluation of XLOC_006390, HOTTIP-005, and RP11-567G11.1 expression in PC cell lines as compared with the HPDE6 cell line. B. XLOC_006390, C. HOTTIP-005, and D. RP11-567G11.1 expression levels in PC tissues were all significantly higher than that in the non-tumorous tissues (n = 144, P < 0.0001). Smaller ΔCT value indicates higher expression. β-actin was used as the normalization control. E. XLOC_006390, F. HOTTIP-005, and G. RP11-567G11.1 relative gene expression was determined using the ΔCT method; data are presented as 2 − ΔΔCT. Fold change > 1.5 was deemed upregulation. XLOC_006390, HOTTIP-005, and RP11-567G11.1 were upregulated in 76.4% (110/144), 81.9% (118/144), and 66.7% (96/144) of PC tissue samples, respectively.