Figure 3. MiR-373 promotes migration and invasion via TXNIP-dependent ROS reduction.
A., C. MCF-7 cells were treated with anti-TXNIP siRNAs A. or miR-373 precursor C. at the indicated concentration. The intracellular ROS levels were determined by DCFH-DA assay. B. MDA-MB-231 cells were treated with miR-373 inhibitor at the indicated concentration. The intracellular ROS levels were determined by DCFH-DA assay. D., E. MCF-7 cells were transfected with 30 nM miR-373 precursor D. or 50 nM anti-TXNIP siRNAs E. following 1 μM H2O2 treatment (non-toxicity) for 12 h, and then the migration and invasion were analyzed (n = 3). F., G. MDA-MB-231 cells were transfected with 50 nM miR-373 IN F. or TXNIP plasmid G. following 2 mM NAC treatment (non-toxicity) for 24 h, and then the migration and invasion were analyzed (n = 3).