Figure 2. MiR-373 induces EMT, migration, invasion and metastasis by directly inhibiting TXNIP.
A., B. MCF-7 cells were transfected with anti-TXNIP siRNAs at the indicated concentration, and then the migration and invasion A. and EMT markers B. were analyzed as indicated (n = 3). C., D. MCF-7 cells were co-transfected with the indicated plasmids and 30 nM miR-373 precursor, and then migration and invasion C. and EMT markers D. were analyzed as indicated (n = 3). E. NOD-SCID mice were transplanted with the indicated Luc-labeled cells (4×106 cells/mouse) via tail vein injection (n = 6), and these mice were then visualized ten weeks post-transplantation using the IVIS 200 Imaging System. F. Histological analysis of the pulmonary metastases in the animal model as described in Figure 2E.