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. Author manuscript; available in PMC: 2016 Jan 28.
Published in final edited form as: Cell Rep. 2016 Jan 7;14(3):403–410. doi: 10.1016/j.celrep.2015.12.054

Figure 1. MCU channel activity is modulated by a mechanism dependent upon matrix [Ca2+].

Figure 1

(A) MCU current density in various bath [Ca2+] (indicated in inset) with 0 Ca2+ (1.5 mM EGTA) in the pipette solution, in response to voltage ramps from −160 to 60 mV. Inhibition by ruthenium red (RuR, 200 nM) in 1 mM bath Ca2+ also shown.

(B) MCU current density measured from MCU-KD cells.

(C) Similar to panel (A), recorded with pipette solution containing 400 nM free Ca2+.

(D) Response of MCU Ca2+current density at Vm = −160 mV, with 1 mM Ca2+ in the bath, as function of free [Ca2+] in pipette (matrix) solution. Data fitted with a biphasic Hill equation (continuous line).