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. 2016 Jan 21;6:19211. doi: 10.1038/srep19211

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(a) Schematic representation of BC2-tagged fusion proteins and fluorescently labeled BC2-Nbs used for co-localization studies. The BC2T sequence was genetically fused to the C-terminus of mCherry-Vimentin (mCherry-VIM_BC2T) and eGFP-PCNA (eGFP-PCNA_BC2T). (b) HeLa cells ectopically expressing mCherry-VIM_BC2T or eGFP-PCNA_BC2T were fixed either with methanol or PFA, respectively, followed by staining with the indicated fluorescently labeled BC2-Nbs and DAPI. Scale bar 25 μm.