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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Mar 26;35(5):1166–1178. doi: 10.1161/ATVBAHA.114.304865

Figure 3.

Figure 3

Mouse lung endothelial cells (MLEC) after Ucp2 silencing and kept at room air or challenged with CoCl2 “IH”. Scrambled siRNA (Sc) was used as negative control. A. Mitophagy associated protein Pink1, autophagy associated proteins LC3BI and LC3BII and mitochondrial biogenesis-associated protein PGC1α in room air after Sc or Ucp2 siRNA transfection. B. Mitochondrial mass in cultured MLEC as assessed by FACS in room air or after CoCl2 “IH”. C. Mitochondrial mass as assessed by FACS in endothelial cells (EC) isolated from Flox or VE-KO lungs in room air and after 5 weeks of IH. *p<0.05. D. Autophagy levels as assessed by immunofluorescent ptfLC3 plasmid. Original magnification of all photomicrographs: ×40.