Figure 6.
Analysis and characterization of serum EVs after hyperoxia. (a) Protein level of serum EVs after hyperoxia. (b) Size of serum EVs measured by DLS after 3 days' hyperoxia. (c) Expression of SP-B, SP-C, and CD9 in serum EVs. SP-B and SP-C are featured markers for lung epithelial cells. (d) Quantification of active caspase-3 in serum EVs and soluble fractions. (e) Expression of caspase-3 in serum EVs after hyperoxia (3 days). (f) Caspase-3 activity in serum EVs. (g) Hyperoxia-induced mouse serum EVs were used to treat peritoneal macrophages (J774). After 24 h, TNF-α and MIP-2 were analyzed using ELISA. (h) Serum EVs derived from the WT or caspase-3-deficient mouse were used to treat J774 macrophages. After 24 h, cytokine productions were determined. Error bars represent mean±S.D. n=3. All other figures represent two independent experiments with identical results. *P<0.05