TABLE 1.
Strains and plasmids used in this study
| Plasmid or strain | Construction/relevant characteristicsa | Reference or sourceb |
|---|---|---|
| Plasmids | ||
| pBP33 | bla PT7-Δ300cdaA | 23 |
| pBP224 | pUT18-cdaR | This work |
| pBP225 | pUT18C-cdaR | This work |
| pBP226 | p25-N-cdaR | This work |
| pBP227 | pKT25-cdaR | This work |
| pBP228 | pUT18-Δ84cdaR | This work |
| pBP229 | pUT18C-Δ84cdaR | This work |
| pBP230 | p25-N-Δ84cdaR | This work |
| pBP231 | pKT25-Δ84cdaR | This work |
| pBP232 | pUT18-cdaA | This work |
| pBP233 | pUT18C-cdaA | This work |
| pBP234 | p25-N-cdaA | This work |
| pBP235 | pKT25-cdaA | This work |
| pBP236 | pUT18-Δ240cdaA | This work |
| pBP237 | pUT18C-Δ240cdaA | This work |
| pBP238 | p25-N-Δ240cdaA | This work |
| pBP239 | pKT25-Δ240cdaA | This work |
| pBP353 | Phelp-lacO-cdaR lacI neo | This work |
| pMAD | bla erm bgaB | 34 |
| pIMK3 | Phelp-lacO lacI neo | 32 |
| pJR26 | Phelp-lacO-cdaA lacI neo | This work |
| pJR27 | bla erm bgaB cdaR | This work |
| pJR28 | bla erm bgaB cdaA | This work |
| pJR29 | bla erm bgaB ΔcdaR | This work |
| pJR31 | bla erm bgaB ΔcdaA | This work |
| pJR40 | bla erm bgaB ΔcdaA ΔcdaR | This work |
| pKT25 | Plac-cyaA-MCS kan | 36 |
| pKT25::zip | Plac- cyaA-zip kan | 36 |
| pUT18 | Plac-MCS-cyaA bla | 36 |
| pUT18C | Plac-cyaA-MCS bla | 36 |
| pUT18C::zip | Plac- cyaA-zip bla | 36 |
| p25-N | Plac-MCS-cyaA kan | 37 |
| L. monocytogenes strains | ||
| EGD-e | Wild type, serovar 1/2a strain | 49 |
| BPL16 | ΔcdaR attB::Phelp-lacO-cdaR lacI neo | pBP353 → LMJR45 |
| LMJR43 | attB::Phelp-lacO-cdaA lacI neo | pJR26 → EGD-e |
| LMJR45 | ΔcdaR | pJR29 ↔ EGD-e |
| LMJR46 | ΔcdaA attB::Phelp-lacO-cdaA lacI neo | pJR31 ↔ LMJR43 |
| LMJR65 | ΔcdaA ΔcdaR attB::Phelp-lacO-cdaA lacI neo | pJR40 ↔ LMJR43 |
a
Designations Δ300cdaA, Δ84cdaR, and Δ240cdaA represent truncated genes lacking the indicated number of nucleotides. MCS, multiple-cloning site.
b
A right-pointing arrow represents a transformation event. A double-headed arrow indicates gene deletions obtained by chromosomal insertion and subsequent excision of pMAD plasmid derivatives (see Materials and Methods for details).