Skip to main content
. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Science. 2015 Dec 1;351(6268):84–88. doi: 10.1126/science.aad5227

Fig. 4. Unbiased genome-wide off-target profile of mutants using BLESS.

Fig. 4

(A and B) Manhattan plots of genome-wide DSB clusters generated by each SpCas9 mutant using the EMX1(1) and VEGFA(1) targeting guides. (C and D) Targeted deep sequencing validation of off-target sites identified in BLESS. Off-target sites are ordered by DSB score (blue heatmap). Green heatmaps indicates sequence similarity between target and off-target sequences.